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Sample information : PLA16



Estimated number of cells
The total number of barcodes identified as cells.
Median UMI counts per cell
Among barcodes identified as cells, the median number of UMI per cell.
Median genes per cell
The median number of genes per cell.
Mean reads per cell
The mean number of reads per cell.
5,597

Estimated number of cell

1,763

Median UMI counts per cell

1,088

Median genes per cell

19,129

Mean reads per cell

Beads to cells


(left) Beads to cells plot
In library, different mRNAs from the same cell will carry the same cell barcode and random unique molecular identifiers (UMIs). However, some mRNA from dead cells mixed in reflection system was inevitability. This graph shows the distribution of UMI counts in each barcode. Barcodes can be determined to be cell-associated based on their UMI counts or by their expression profiles. Therefore, the graph contains both cell-associated (Colored Regions) and background-associated barcodes (Gray Regions).
(right) Histogram
Histograms of number of beads per droplet.
Summary


Estimated number of cells
The total number of barcodes identified as cells.
Mean reads per cell
The mean number of reads detected per cell.
Mean UMI counts per cell
The average number of UMI counts per cell. This item refers the average abundance of expression in each cell.
Median UMI counts per cell
The median number of UMI counts per cell.
Total genes detected
The total number of genes detected.
Mean genes per cell
The mean number of genes detected per cell. This item refers the activity of gene expression in each cell.
Median genes per cell
The median number of genes detected per cell.
Sequencing saturation
The fraction of UMI originating from an already-observed UMI.
Fraction reads in cells
The fraction of uniquely-mapped-to-transcriptome reads with cell-associated barcode.
Plot
The distribution of nFeature and nCount in violin.
Sample name
PLA16
Species
Homo_sapiens
Estimated number of cells
5,597
Mean reads per cell
19,129
Mean UMI count per cell
2,934
Median UMI counts per cell
1,763
Total genes detected
30,977
Mean genes per cell
1,425
Median genes per cell
1,088
Fraction reads in cells
30.48%
Sequencing saturation
76.51%
Sequencing


Number of reads
Number of raw off-machine reads obtained by this sequencing program.
Reads pass QC
Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
Reads with exactly matched barcodes
Number of reads with barcodes that match the barcode whitelist before correction.
Reads with failed barcodes
Number of reads with invalid barcodes that fail to match the barcode whitelist.
Reads filtered on low quality
Number of reads in low quality. The QC will filter the average base quality < Q20 or 2 bases < Q10 in the first 15 bases.
Q30 bases in cell barcode
Fraction of cell barcode bases with Q-score ≥ 30.
Q30 bases in UMI
Fraction of UMI bases with Q-score ≥ 30.
Q30 bases in reads
Fraction of RNA read bases with Q-score ≥ 30.
mRNA
Number of reads
566,177,609
Reads pass QC
90.52%
Reads with exactly matched barcodes
86.45%
Reads with failed barcodes
7.75%
Reads filtered on low quality
0.16%
Q30 bases in cell barcode
95.53%
Q30 bases in UMI
94.22%
Q30 bases in reads
88.39%
Droplet index
Number of reads
132,729,966
Reads pass QC
95.74%
Reads with exactly matched barcodes
90.35%
Reads with failed barcodes
4.26%
Reads filtered on low quality
0.0%
Q30 bases in cell barcode
98.17%
Q30 bases in reads
98.00%
Mapping & Annotation


Reads pass QC
Number of reads after quality control (QC) that can be used for downstream analysis. QC includes filtering low quality reads and invalid barcodes.
Reads mapped to genome
Fraction of reads that mapped to the genome.
Plus strand
The number of reads in plus strand.
Minus strand
The number of reads in minus strand.
Mitochondria ratio
The fraction of reads mapped to mitochondria.
Mapping quality corrected reads
The number of reads that align to a single exonic locus but also align to 1 or more non-exonic loci.
Reads mapped to exonic regionse
Fraction of reads that mapped to an exonic region of genome.
Reads mapped to intronic regions
Fraction of reads that mapped to an intronic region of genome.
Reads mapped antisense to gene
Fraction of reads mapped to transcriptome, but on the opposite strand of their annotated gene. This part of reads will be filtered out cause them are opposite to theoretical direction.
Reads mapped to intergenic regions
Fraction of reads that mapped to intergenic regions of genome.
Reads pass QC
512,519,770
Reads mapped to genome
86.69%
Plus strand
61.23%
Minus strand
38.77%
Mitochondria ratio
0.91%
Mapping quality corrected reads
0.28%
Reads mapped to exonic regions
27.3%
Reads mapped to intronic regions
35.8%
Reads mapped antisense to gene
8.5%
Reads mapped to intergenic regions
36.9%
Include introns
True
Cluster


Left
This plot shows that the total UMI counts for each cell-barcode. Two-dimensional horizontal and vertical coordinates of each dot are obtained using the uniform manifold approximation and projection (UMAP) algorithm. Each dot represents a cell and is colored according to UMI counts. Cells with greater UMI counts likely have higher RNA content than cells with fewer ones.
Right
The figure is automated clustering each cell-barcode by UMAP algorithm. The cells clustered into the same group have similar expression profiles. Each dot represents a cell, and is colored according to different cluster.
Marker


Table
The table shows the top 30 differentially expressed genes for each cluster. Here a differential expression test was performed between each cluster and the rest of the sample for each gene. The p-value is a measure of the statistical significance of the expression difference, the smaller the P value, the closer to the theoretical value. Avg_log2FC is log fold-chage of the average expression between the markers for every cluster compared to all remaining cells.
gene cluster p_val_adj p_val avg_log2FC pct.1 pct.2
PAPPA01.4284755135757389e-831.6790449949955792e-870.6981.00.997
PLCG202.16828510389414e-092.276771570127452e-110.6690.4590.436
PSG404.072708931562322e-461.4361313475712455e-490.6050.8610.848
CSH209.688610768565083e-1657.592062663922802e-1690.6031.01.0
CSH102.1284740402599128e-1878.339435177134008e-1920.5971.01.0
PSG103.909178345094605e-679.18977787508037e-710.5780.9610.961
PAPPA203.7349610538736094e-262.4877302008326358e-290.5740.980.967
PLAC406.862888751251328e-294.033355454639734e-320.5510.9630.94
KISS101.760391854609542e-446.897276396229056e-480.5360.9210.923
AFF105.1118453114436184e-207.009935583611905e-230.5350.9730.948
CGA01.0271953142502396e-603.2196695192578916e-640.5240.9920.984
INHBA01.0548832075776082e-143.1824631502360945e-170.5230.5790.566
SLC26A302.3088369026444887e-784.5230515665174326e-820.5030.9980.997
CRH01.554326598447106e-289.743848910846569e-320.4970.7760.777
S100P01.9584235983770242e-101.542307500191129e-120.4970.5040.514
CYP19A102.451400375098617e-252.0169810710759296e-280.4950.9980.993
PSG903.7685634161581186e-223.838994194260513e-250.4880.7030.705
PDE4D01.1396813554694268e-062.7015132235983356e-080.4880.530.517
ADAM1204.7600372584860835e-122.3498988934265038e-140.4731.00.999
TFPI208.364861687646277e-622.2941672927760816e-650.4710.9910.993
RASGEF1B07.098666062974322e-821.1125128022527636e-850.4681.01.0
PSG302.4302939525166003e-331.2378568891868434e-360.4560.8670.876
ADGRL300.0150153341146667920.0014107577952031880.4540.3270.306
PSG502.4147914489259423e-341.1353484068139054e-370.4510.8810.892
GULP107.694760001206946e-142.7434986487083496e-160.4510.9190.884
FBLN107.531611034777822e-373.246002483350548e-400.4290.9420.946
PSG609.619958958568316e-181.8091839909543518e-200.4230.7090.715
COBLL102.2679707377230746e-101.8216275564519466e-120.4110.9680.936
HSD3B101.0673168744950733e-152.592706430227424e-180.4050.6750.682
EXPH501.2352365682728662e-134.646111764063596e-160.3990.7730.767
CHODL02.2449477911676928e-074.116426620171924e-090.3970.610.6
PSG206.276842395214664e-264.9185772794849074e-290.3950.860.874
CD6306.617191400701311e-071.4363217631111256e-080.3820.4830.505
LINC0194901.597051989721666e-072.8345592263602033e-090.3760.9270.897
GDF1505.220446484852685e-225.727089353754463e-250.3730.8150.83
EPS8L101.1575992946080037e-071.948239817237686e-090.3690.5640.583
HSPB101.044531290607858e-053.345989700557152e-070.3560.4680.488
GDPD500.0094697742384749310.00082257138607134440.3220.4060.432
NEAT105.632577908218662e-151.56687313984847e-170.3210.9770.981
PSG1106.649718346212773e-062.0113554689010934e-070.3210.5090.538
TIMP203.983060599694034e-151.0924038787704518e-170.3180.770.797
GH201.1587778751003966e-081.4710027486287995e-100.3170.5880.617
GRIP104.1402621007200214e-112.579248810933211e-130.3090.960.948
EPS8L200.00232572234268120640.000162493865803150270.3070.4780.51
BMP101.6869060144845624e-055.809624208486191e-070.2820.5350.57
GPC302.2242954586829648e-111.2810854226634637e-130.2740.8210.838
RYBP04.2416567151788784e-078.708334125117472e-090.2690.7130.722
SDC104.2020947146271735e-094.7910103120969614e-110.260.6390.682
VGLL305.013780716139851e-087.739801755902917e-100.2580.7980.807
LNPEP02.528093707469031e-083.4965210936667627e-100.2410.6770.712
KLRD118.959334753101267e-1893.51029845750941e-1932.60.8510.439
PSG8-AS111.1593168900953182e-166.359141347543178e-192.5620.2350.056
LINC0148311.103674656662848e-827.783624111558697e-862.2550.6180.289
LVRN14.072630012705652e-1191.1169694036335681e-1222.2060.7610.388
LINC0229111.0454559652736371e-571.269801156740303e-602.1190.5130.208
ENSG0000025040710.070043485463635620.00247812825191642652.0820.0870.025
ADAMTS614.766275705579404e-1365.602330100982727e-1402.0190.8560.567
ENSG0000028890312.4402659789655176e-102.2850901891343445e-122.0150.2060.065
SYT1612.329669519222939e-063.2403427470287326e-081.9360.1650.053
SLC26A712.105419437990418e-072.507728359319387e-091.9090.1840.064
POSTN17.819202697845417e-282.267057162718179e-301.8440.3760.154
LINC0160511.6763547718398994e-121.3333072863045081e-141.8320.2410.086
COASY15.103509102436132e-162.999358873821337e-181.8210.2810.11
LINC0261512.8568750466516527e-612.7983339014336608e-641.7660.6060.32
MICU312.20505606906162e-801.727897244886275e-831.730.730.421
RHOBTB119.198214890865303e-1271.8019462623643974e-1301.7020.8890.673
NECTIN3-AS112.1996506802558583e-072.6285838556519093e-091.6930.1920.072
ENSG0000025158416.286902969631162e-144.2367563012833915e-161.6860.270.108
ADGRG614.675295644557927e-902.3813361822377087e-931.6810.7820.513
NIM1K10.0114974255320489170.00031623213272336091.6790.1150.042
ENSG0000027373410.0026189645539601885.9207089591154195e-051.6560.1310.05
SPTLC315.279287454762864e-1111.6547545209459277e-1141.6540.8680.618
ENSG0000028607211.298349452886337e-121.0123086671801162e-141.6430.2630.109
ENSG0000026692913.9264522491308083e-281.0922623112027873e-301.630.4160.194
LINC0088213.327629999176515e-505.084730242051643e-531.6240.6080.353
ENSG0000029059811.4962092801028248e-052.3390177595150536e-071.5950.1720.068
BCL614.275695493314902e-123.501235583994101e-141.5910.2640.114
MIR181A2HG11.5337876146481663e-062.049216693159208e-081.5580.1910.077
CSGALNACT115.743685957078284e-745.17591102193318e-771.5350.7960.551
PHYHIPL14.884633821811007e-507.655265951198538e-531.5230.6250.358
FAM107B14.0780851285042676e-604.633642938785557e-631.5170.6880.42
FNDC3A13.010017991203829e-1334.717341991464685e-1371.510.960.787
DYSF13.569396613696747e-603.9158055551270977e-631.5070.6920.424
ENSG0000028542418.486671068988629e-117.581244382437047e-131.5070.2640.125
THSD418.143920450682964e-491.3401428473482133e-511.490.6270.376
SESN311.2292764920957669e-176.309415839225226e-201.4860.3510.177
LINC0047411.18499856107137e-312.878576608800883e-341.4840.4880.264
ENSG0000022626610.0072004116585264470.000188170457087220951.4820.1310.056
BAZ2B14.6623115183787225e-763.8360906588548826e-791.4620.7930.553
ENSG0000023049013.831475721020851e-397.656045988796906e-421.4380.5690.332
ENSG0000028732614.97953463517723e-058.447825479104104e-071.4340.1730.073
ENSG0000024925812.2907404779747094e-053.6888074930361067e-071.410.1810.078
ENSG0000028294311.42843404741502e-215.8764868933345254e-241.4050.4250.245
IGSF512.463877287854976e-092.452003413059452e-111.4020.2430.106
FAM156A10.000118778965524459712.136094705784077e-061.3920.1730.077
DAPK111.1993950811324682e-847.5188345014769e-881.3650.8970.722
FAM13A11.7903099198025626e-879.820294979914538e-911.3510.8990.712
ENSG0000019821110.062836832212301810.0021689946001268621.3420.1090.046
SWT114.694469305938178e-123.8625496777299586e-141.3370.2960.144
NCF4-AS110.0161241818356003020.000463705264558657771.3320.1260.054
CPS1100.0101092758488157962.3369011287079574e-056.8540.6250.021
SLC1A2100.0023947289056457913.2152685272877255e-066.7760.6880.018
ADCY5100.0101164824702376072.3782037699888587e-056.620.6250.017
FAR2101.8101698903520602e-063.5461542341262004e-106.220.9380.104
GREM2100.000131689046726143456.191547077983472e-085.6810.8120.084
ERVFRD-1100.0032317035022059545.318009132650945e-065.5660.6880.036
SH3BP4100.00089898959558283258.453453862785715e-075.3470.750.066
IGSF5108.395646465461314e-079.868330288909589e-115.051.00.129
SLC22A11104.028288136463831e-061.1048080929062734e-095.0380.9380.134
MTSS1101.605957746353407e-062.516879279635477e-104.8361.00.223
BLNK100.072192394850264280.00027110531144418464.6630.5620.038
SLC13A4100.000383289329493042642.552959527242771e-074.5040.8120.144
SLC52A1100.083764479890515340.000331474061393333464.4330.5620.057
GRAMD1C100.0082469294412974311.7125230591574806e-054.220.6880.066
PIK3C2A102.86675210689613e-066.739220562385605e-104.1290.9380.301
GNA12100.00100285923616121.060894071086957e-064.1160.8120.246
TACC2104.968758049719679e-071.94677665232131e-114.0531.00.825
STON2100.0124440962587051933.217922474139179e-053.9530.6880.118
ATG9B100.000217101013388516761.1908530295965342e-073.940.8750.269
TBX3-AS1100.038219224872432010.000125785169818763033.9210.6250.089
CPM100.00097130667704373849.514033195977535e-073.8180.8120.272
ERVV-1100.000194167486692931759.88981439097329e-083.7670.9380.224
ENSG00000289084100.00084332426039094297.291711848988342e-073.7670.8750.372
NEDD4L100.0088724805575246381.9119477751982724e-053.7380.750.207
LCMT1-AS2105.84423518205798e-061.868146957603105e-093.6471.00.459
KMO100.00060202324700817314.639199556357506e-073.6160.8750.243
APMAP100.0163634426760939574.616102623824648e-053.6080.6880.13
FAM214A100.019766909159149925.8085577202376055e-053.5990.6880.146
ABTB2100.00560772773007971.032649779860306e-053.5830.750.197
FGD6100.0029880842465249724.331744588074441e-063.550.8120.166
AK3100.0073581868032289551.470311197604814e-053.4860.750.17
ENSG00000236283100.078122545236361020.00029996510728219173.4680.6250.118
EIF2AK1100.0074519224623604961.5182383263830498e-053.4630.750.158
ATG2B100.065975175291773030.000242983445418903153.4630.6250.09
CNNM2100.0030386563824322734.609537868693196e-063.4160.8120.267
DYSF105.84423518205798e-062.0608124686957575e-093.4151.00.471
EPB41L3100.0030386563824322734.724995553040979e-063.3660.8120.388
PBX1100.0018635359702838552.1904195865891804e-063.3580.8750.344
SAMD8100.083764479890515340.00033084797239972063.3360.6250.123
RIN3100.0104981426995261252.5090573391493695e-053.3150.750.189
SLK100.010526031707335592.556964172921704e-053.2970.750.143
SLC40A1100.035980789076534220.000116577267947071133.2910.6880.177
ARHGAP24100.00084332426039094297.599599572539155e-073.2370.9380.372
BRAF100.0023947289056457913.2839208438507496e-063.1960.8120.411
ATP10D100.00053376706044400613.7643721694127295e-073.1740.8750.425
VEZT100.0042002868908536047.405591430804066e-063.1510.8120.237
WDR26100.0059959052090545841.1415943185448209e-053.0690.8120.234
CSF3R108.395646465461314e-076.95196436454496e-113.0371.00.667
APP100.00176676460357792172.0074510638937323e-063.030.8750.379
KCNN4100.009255815282036612.067082518027218e-053.010.8120.184
ENSG00000284931115.224126252549429e-071.0234154003348801e-1011.1241.00.047
HBG2115.224126252549429e-079.769532456635685e-1110.7821.00.137
HBG1115.224126252549429e-079.78089259467327e-1110.7691.00.081
HBA1115.224126252549429e-079.849325121721672e-1110.5061.00.121
HBA2115.224126252549429e-079.74685086571118e-1110.3641.00.241
ALAS2110.093173073632497923.285498031941705e-0510.2720.6430.003
HBB119.093771128005677e-062.1377826575259205e-0910.0120.9290.045
CHODL21.1936360533331472e-1059.35341498517531e-1101.930.8730.552
ENSG0000022871420.0047772961489016120.000101449457849965661.8810.1260.042
ACOXL29.280768601513726e-499.817840858867321e-521.8730.5530.25
ENSG0000027171729.08432947007047e-163.701642694382827e-181.7670.3070.124
ADGRL322.3438618351532256e-433.0304995713692136e-461.7610.5560.265
CTDSP124.810573669357066e-436.408318174122957e-461.6140.5820.304
EXPH524.422410878384681e-1061.7327159340142933e-1101.560.9490.735
DEPDC1B22.119726652943679e-778.305162609974058e-811.5360.8830.613
THSD7A21.5638967993253801e-747.352882338245725e-781.4760.8720.641
PLCL221.9453451136697244e-729.908508591351493e-761.3650.9740.775
SCIN24.299883752943182e-682.527064071392381e-711.340.8710.65
LINC0194921.385660175453941e-1021.6287194006824522e-1061.3280.9950.887
PLCB125.534021030141092e-525.2037967607015714e-551.3090.8020.563
GRAMD2B24.5216745762678604e-543.8980324879580656e-571.3030.8350.597
IGF2BP223.6663078072698403e-376.033182929331712e-401.2920.6770.435
BMP122.6976164531164516e-443.2765000214163693e-471.2640.7650.523
ENSG0000024467626.694253068512744e-133.4883660154064766e-151.2640.3520.185
ENSG0000026163221.248009830738796e-589.290517095967216e-621.2590.8910.703
NHSL126.926964213127513e-693.7996120747476853e-721.2570.920.721
LAMA320.00094188316504386861.686481238197108e-051.2550.1810.089
GULP121.2462828721874058e-951.9531918225716505e-991.2540.9820.876
GDPD522.5207611419881495e-315.03697912633294e-341.250.6250.388
CERS421.441035499659016e-071.3663385609743442e-091.2340.2690.142
RYBP21.6041819435152145e-581.2570481083847625e-611.2240.8890.688
ARHGAP4221.207607782819654e-381.8452651933537007e-411.2140.7750.541
ENTPD122.5679995355151352e-208.753514852870617e-231.20.490.299
OSMR-DT21.2334727689651321e-117.297511582248753e-141.1730.3540.197
LINC0111920.0012560870531139662.3475043072341095e-051.1690.1890.099
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PDE4D71.9161420709697324e-441.5015022301216413e-483.2810.8810.509
DDR270.078766952765113150.000481434182163446753.140.1880.026
ENSG0000028555870.067188912875669420.00040277019433363713.0470.1940.031
ENSG0000026182170.000147165841313412482.488291865452367e-072.6770.3120.088
ADGRL376.3155046379387e-181.4846619843918112e-212.4770.650.3
LINC0158870.0046142130101298531.3529695210380217e-052.3990.2690.075
ENSG0000027421370.014847259410380975.584519466350246e-052.3130.2560.075
ENSG0000028913070.041038632960279060.000210635932993635432.2290.2440.083
LYN72.172396112600396e-081.702304676253102e-111.8740.5120.248
TENT5A72.6163635861723847e-177.175702348159186e-211.8090.7620.522
ATG9B78.046906294734791e-078.197295132355309e-101.7120.50.264
ARRDC3-AS170.03471305053670690.000161848255058892821.6550.2940.128
NEAT172.534305441942708e-499.929496696872264e-541.6511.00.98
TLE579.264233832444615e-105.807614360346113e-131.490.6440.456
ANKRD1270.0014151751773448343.3268232825565193e-061.4740.4060.235
NECTIN377.778089357014192e-143.04748241077232e-171.3180.8440.661
COBLL171.2699050058644665e-281.4926595688568742e-321.2881.00.942
BACE279.945237774375694e-124.675894420425041e-151.2670.7560.613
RNF1170.0969526670923020.00064103991719178561.2610.3250.195
SLC27A670.063669931967000760.00037419150550680221.2420.350.2
PCBP270.0276158719525049220.000117937940007955041.1840.3880.241
JUND70.00317600387420499078.461711532576083e-061.1770.4750.309
IGF2BP273.419957746442272e-064.421839346181678e-091.1680.6250.468
TRIM2570.00168159088968273874.0190042029011895e-061.1380.5190.353
XPO170.0021757597764659265.370562469825387e-061.1050.5060.368
ENSG0000026097170.036131682768494670.000175467063408911731.0960.4250.298
ZNF29277.422095112791282e-104.3620039451423903e-131.0810.8060.666
ZNF9174.943883369609018e-084.067764399239485e-111.0650.7440.615
CDYL71.5092274289349506e-071.4191693098161977e-101.0580.7880.625
TPTEP170.087045489211989170.00054567559745791121.0180.4120.315
INHBA71.0437003206201469e-051.4721314713131406e-081.0080.7060.564
YTHDC170.072139717468176740.000435274712616041130.9960.4190.307
ENSG0000028871471.851881544974757e-081.306032512226056e-110.9570.8690.761
EXPH578.109949845013933e-086.990514304364946e-110.9480.8750.766
AEBP270.00042781386410455048.045709938885875e-070.9410.6310.516
ENSG0000027316770.07767274678542040.000471703003241788260.9380.4310.302
PAK170.0082406630080470432.6798379096027292e-050.9280.5250.397
SLAIN270.055486366909713230.000319574342190488770.9240.4560.338
ARHGEF1580.000268626213260082444.041549421771409e-067.4090.2180.002
EGFL787.611813120136874e-591.1929339215823961e-627.3450.7950.024
FCGR2C80.026955360209033740.00070654452767478637.2880.160.001
SHANK383.479232084075023e-191.0223814062047045e-217.0040.4550.007
MEOX285.614589141736899e-354.3996310321959794e-386.9550.6150.016
PCAT1986.375954651421321e-066.694964724291479e-086.7630.2560.003
ADCY484.998326657246399e-056.208006701199344e-076.6870.2370.004
ZNF36680.00029540550887052474.525469340139292e-066.650.2180.003
SLC9A3R280.094246083860243260.0030279273112643296.5450.1410.002
MECOM81.1669130600565926e-394.8436306606343805e-436.5410.660.028
ZNF52184.288912655125489e-315.041232600155337e-346.5220.5830.019
LDB286.366950451432263e-674.989186577935401e-716.430.8590.07
PLCB481.1669130600565926e-395.029206465001182e-436.4210.660.022
RALYL86.172914124107716e-383.869710691757374e-416.3430.6470.03
ERG81.239218272255254e-212.8646271231070006e-246.3420.4870.013
RASIP180.0028892449230976365.626120466949517e-056.3150.1920.004
ENSG0000022930880.00284034875609393655.508649587691489e-056.30.1920.003
NRG384.3206257230664585e-161.5404809027114669e-186.2460.4230.015
DIPK2B82.1236763544020122e-101.3229813907061081e-126.210.340.008
BMP683.488152179360279e-112.0363384975303907e-136.1980.3530.009
HLX80.0006238607333637881.0192764401234167e-056.1230.2120.005
ROBO480.0122231086165080270.00029069572269013726.1130.1730.003
RAPGEF581.5704558662668482e-223.1996123122625127e-256.1110.50.016
ST6GALNAC380.00011267861889908871.5495903786224242e-066.0420.2310.006
IDO284.845012063086466e-191.4616852597957054e-216.0320.4620.021
PTPRB86.148118692714208e-221.348958377902267e-246.0090.4940.018
PECAM186.66204663168272e-597.830638990341324e-635.9880.8140.067
RHOJ80.0029671525500788575.8127033461561275e-055.9580.1920.004
DACH188.993021056743601e-301.3036938412393263e-325.9330.5770.027
PALMD81.0357762062932837e-078.562817048461499e-105.8940.2950.008
LRRC3689.55792776468696e-061.0448073683922979e-075.7970.2560.008
HSD17B284.893067581766919e-411.7254087778044221e-445.7940.6860.052
MYZAP82.3535957139895197e-081.8074080630879829e-105.7840.3080.01
JAM282.8169157378436177e-121.412707026775783e-145.7670.3720.012
ZEB181.4726598114487813e-242.481070873028155e-275.7490.5260.023
TIE180.00201196312021658443.72074831619413e-055.7390.1990.006
ENSG0000027311289.640301827179792e-061.057589041887843e-075.7230.2560.008
RASGRF286.943733246074446e-172.366903547421842e-195.6790.4360.015
STXBP688.650568841063204e-375.761848932260098e-405.6490.6470.037
KANK381.2962591013197664e-071.0817818774482242e-095.6390.2950.01
MMRN187.120307094933866e-059.122518591244658e-075.5950.2370.007
CGNL182.488102551536891e-206.531476352817917e-235.5190.4810.023
CD3483.735914586167801e-102.3859032149251716e-125.5140.340.012
ENSG0000022619782.3547421771752377e-052.7862404008420707e-075.4720.250.01
MAGI185.493823169394033e-722.152498988909624e-765.470.910.191
ADGRF580.000424935152416980176.6596427131133525e-065.440.2180.007
BICD183.648055710471596e-411.1434567129166935e-445.4170.6920.067
CACHD181.0795003544407027e-115.921327807142514e-145.4090.3650.015
KDR85.836618007566709e-066.105775214591981e-085.3570.2630.009
FCGR2B80.00014797715450298052.08720666148466e-065.3490.2310.009
PTPRC92.877963986954059e-051.0148366525324816e-085.9280.5880.027
PRKCB90.0035728163009979425.976086582000005e-065.2860.4710.026
AOAH94.844593141253575e-052.3748795460564957e-085.2350.5880.045
APBB1IP90.0047361867919612499.463837573562032e-064.7190.4710.036
ARHGAP1590.033418227133180670.000109984370144073034.6930.4120.034
CD7490.0147691963734684774.339966806449617e-054.6350.4410.043
DOCK290.00072492699287859277.952809544567878e-074.5550.5290.044
PLXDC299.328521812856492e-057.67538918113021e-084.5360.5880.088
CAMK1D90.00097356673317250721.277535897995713e-064.2760.5290.055
HLA-B90.058548997214778530.000213339213296806924.1210.4120.059
SAMHD190.0082267792168461441.9339683932561558e-054.0440.4710.058
CELF290.0092807358052430512.348771668938251e-053.940.4710.061
LDLRAD490.0133763361931962693.878262266569462e-053.6540.4710.068
B2M92.966002893246337e-065.81045114846675e-103.5940.7350.209
FYB190.088007179360441740.00036205594298657613.5260.4120.074
ABR90.083877575137853890.00033419404903454773.5030.4120.068
ZSWIM690.0035728163009979426.019319866117287e-063.4230.5290.103
DPYD90.000476694847176685634.856038093716971e-073.2120.6180.187
ZEB290.0035728163009979425.699941794482466e-063.1830.5590.104
LRMDA90.0061813267551639591.3336518318295374e-053.1570.5590.145
DOCK490.035936523147572740.000119680463407267263.1420.4710.096
CTSD90.0248783113342582847.797926994243086e-052.9380.50.14
GPCPD190.0211087794845187636.368279670524409e-052.8950.50.124
FNBP190.004435406008321047.919648972456798e-062.750.5880.177
ACTB90.053878728581170390.000189988856024187422.690.50.166
MBNL190.000291634954956047542.8565896931791677e-072.6640.7060.289
ATXN190.0879017719750150.000358178281761609552.5860.4710.128
DLEU290.00517235118009081.0740689282012788e-052.4820.6180.223
CYRIB90.09419544493384480.000401245645561277932.4030.50.19
FOXN390.00155576751962460182.255353924934775e-062.3950.6760.333
RNF21390.00664412782460612351.5098515606596213e-052.2380.6470.264
EIF4G390.083877575137853890.000335207956120405032.060.5590.227
LYN90.058075618640391370.00020933890666912222.030.5880.254
ANKRD1190.0046180776649553669.04689430113107e-061.9070.7060.396
DOCK890.053878728581170390.000189861148296300031.7830.6470.358
MALAT198.451756466374891e-055.960569540992361e-080.8041.01.0
Cell Annotation


Predicated cell type
Predicted cell types were assigned using scHCL for the human dataset and scMCA for the mouse dataset, relying on Pearson correlation with the Human Cell Atlas and Mouse Cell Atlas, respectively. UMAP plots illustrate cell-type identities for all cells, with colors representing specific cell types. These annotation results are provided for reference purposes, assisting in the evaluation of library quality to determine acceptability.
Saturation


Left
The plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.
Right
The plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.